|
KMID : 0364820080440040346
|
|
Korean Journal of Microbiology
2008 Volume.44 No. 4 p.346 ~ p.351
|
|
|
Development of Human Papillomavirus DNA Array by Using Lateral Flow Membrane Assay
|
|
Kim Ki-Whang
Lee Hyung-Ku
Cho Hong-Bum
|
|
|
Abstract
|
|
|
|
This study develops DNA array which can detect specific sequence of human papilomavirus (HPV) by using lateral flow membrane assay which is usually used for point of care test including pregnant diagnosis. Principle of HPV DNA array is as follow; fixing DNA probe which is peculiar to HPV type 6, 11, 16, 18, 31, 45 on a surface of lateral flow membrane and inducing hybridization response between probe and HPV PCR products which is obtained by using biotin-labeled MY09/11 primers. And then, we can see the result of DNA hybridization that streptavidin labelled colloidal gold is responded with hybrid biotin. Lateral flow membrane array developed in this study confirms major HPV type economically and conveniently compared with existing HPV DNA chip method.
|
|
|
KEYWORD
|
|
|
DNA array, human papillomavirus (HPV), lateral flow membrane
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|